Molecular Basis of Inheritance
1. What experimental
evidence led scientists to believe
that the genetic information is encoded within DNA?
Be familiar with the classic experiments carried out by Griffith,
and Hershey and Chase which led to the conclusion that DNA rather than
protein serves as the carrier of genetic information.
2. What experiments
led to an understanding of the
structure of the DNA molecule? Discuss the contributions made by
Chargaff, Wilkins, Franklin, and Watson and Crick.
3. Describe the
molecular structure of the DNA molecule
in detail (i.e. the Watson and Crick Model). What forms
“backbone” of the molecule? What is responsible for holding the
strands of the double helix together? What do we mean by antiparallel
strands? Why (chemically) do the base pairs pair as they do
adenine with thymine and guanine with cytosine)?
4. Describe in detail
the process of DNA replication.
- Is DNA replication conservative or semi-conservative?
the experimental evidence that was performed to suggest that DNA
- Discuss the functions of the various enzymes involved in
polymerases, nucleases, primases, DNA ligase, helicases).
- How does elongation of the new strand occur? In what
DNA replication occur (i.e. 5’ to 3’ or 3’ to 5’ on the growing strand)?
- How is DNA replication on the leading strand different
on the lagging strand?
- What is DNA priming? Compare priming on the leading strand
- Where does the energy come from to add new nucleotides to the
- Why is DNA replication such an accurate process? How does
of the DNA molecule take place? How is damaged DNA repaired?
- What do we call it when an uncorrected error that occurs duing